DNA Methylation Sequencing Analysis
  • Introduction
  • Data Preparation
    • Locate the MethPipe Files
    • Download Utilities
    • Download Annotations
    • Annotation File Preparation – Defining Genomic Regions
  • Analysis Work Flow
    • DNA Methylation at Genomic Bins
    • DNA Methylation at CpG Islands
    • DNA Methylation at TFBS
    • DNA Methylation at Various Genic Structure Regions
    • DNA Methylation at Repeat Elements
    • Add CpG Islands Co-localization Information to HMR BED Files
    • Similarity and Differences of HMRs and PMDs from H1 and IMR90
      • HMRs
      • PMDs
  • Visualization Using R
    • Install R Libraries
    • Execute the R Scripts
  • An introduction of UCSC Genome Browser
    • General Usage
    • The Compressed Binary Index Format
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  • Things you need to know before using the ALPS server:
  • Things you need to know if you are performing this tutorial on your own computer/server:

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Data Preparation

First, log into your account at alps1.nchc.org.tw with the user name and password provided. As show in Table 1, you will find four folders in your home directory.

Table 1. Folders in the home directory

Folder name

Description

Data

Downloaded public data

Output

Processed files

Scripts

R scripts and output PDF files

Tools

Executables

Things you need to know before using the ALPS server:

  1. Users must submit your jobs to LSF via the bsub command. The escape character (or backslash \) was added before special character to make the command compatible.

  2. The allowable space for each account is 1GB.

Things you need to know if you are performing this tutorial on your own computer/server:

  1. Most of the commands are written for batch job submission to the LSF system. You will need to modify the commands if you are not using LSF.

  2. You may create the folders as listed in Table 1 in your home directory, or other locations and remember to change the file paths in commands.

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Last updated 5 years ago

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