📄
Guide to RNA-seq Analysis
  • Introduction
  • Preparations
    • Work environment
    • Softwares and databases
    • Obtain sequencing data
    • R packages
  • Raw read processing
    • QC & trimming
    • Mapping & quantification
      • Alignment-free method
      • Alignment-based method
  • Differential expression analysis in R
    • Building a TxDb object
    • About tximport
    • Convert Salmon output to Sleuth-compatible format
    • Differential gene expression (DGE) analysis using DESeq2
      • DGE analysis with Salmon/Kallisto input
      • DGE analysis with STAR input
      • DGE analysis with STAR + RSEM input
    • Differential transcript expression (DTE) analysis using DESeq2
      • DTE analysis with Salmon/Kallisto input
      • DTE analysis with STAR + RSEM input
    • DGE and DTE analysis of Salmon/Kallisto inputs using Sleuth
    • Differential transcript usage (DTU) analysis
      • DTU analysis using DRIMSeq
      • DTU analysis using DEXSeq
Powered by GitBook
On this page
  • List of R packages used
  • CRAN
  • Bioconductor
  • GitHub

Was this helpful?

  1. Preparations

R packages

PreviousObtain sequencing dataNextQC & trimming

Last updated 4 years ago

Was this helpful?

List of R packages used

CRAN

  • data.table:

  • dplyr:

  • reshape2:

  • ggplot2:

  • ggrepel:

  • ggbeeswarm:

Bioconductor

  • GenomicFeatures:

  • tximport:

  • DESeq2:

  • apeglm:

  • EnhancedVolcano:

  • DRIMSeq:

  • DEXSeq:

  • stageR:

  • edgeR:

GitHub

if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("devtools")    # only if devtools not yet installed
BiocManager::install("COMBINE-lab/wasabi")
if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("devtools")    # only if devtools not yet installed
BiocManager::install("pachterlab/sleuth")

wasabi:

sleuth:

https://cran.r-project.org/package=data.table
https://cran.r-project.org/package=dplyr
https://cran.r-project.org/package=reshape2
https://cran.r-project.org/package=ggplot2
https://cran.r-project.org/package=ggrepel
https://cran.r-project.org/package=ggbeeswarm
https://bioconductor.org/packages/GenomicFeatures/
https://bioconductor.org/packages/tximport/
https://bioconductor.org/packages/DESeq2/
https://bioconductor.org/packages/apeglm/
https://bioconductor.org/packages/EnhancedVolcano/
https://bioconductor.org/packages/DRIMSeq/
https://bioconductor.org/packages/DEXSeq/
https://www.bioconductor.org/packages/stageR/
https://www.bioconductor.org/packages/edgeR/
https://github.com/COMBINE-lab/wasabi
https://github.com/pachterlab/sleuth